Levitate is a spin-out of Cyrus Biotechnology. Cyrus, initially established as a spin-out of the Baker Lab where Rosetta originated, was dedicated to developing Bench, an intuitive online version of Rosetta. While Bench development was halted in 2019 to prioritize protein therapeutics advancement, Cyrus continued to refine Rosetta and AI protocols internally via APIs to help model their therapeutics. In 2024, Cyrus underwent a restructuring, resulting in two distinct entities: one focused on therapeutics development under the Cyrus banner and the other, Levitate Bio, solely managing software, services, and IT operations. Levitate Bio’s inaugural client became Cyrus, subscribing to software access and utilizing Levitate’s IT services.

Our Protocols

De Novo Design

RosettaFold Diffusion
- Hallucinate novel structures from scratch to create new folds, binders, and linkers that are highly stable.
ProteinMPNN
- Hallucinate novel sequences that are favorable for your protein structure and highly stable.

Mutation Sampling & Design

Rosetta Design
- Redesign target regions with physics-based sampling of combinations of mutations
Disulfidizer
- Scan structure for favorable disulfide
Point Mutation Scan
- RosettaDDG or ThermoMPNN (AI)

Protein Structure Prediction

Homology Modeling
- Template-based modeling with Rosetta optimization
Artificial Intelligence
- AlphaFold2, OpenFold, ESMFold, and AlphaFold2 Multimer

Protein Structure Optimization

Relax, Prepare, Loop Model, Minimize, or Repack
- Rosetta’s physics-based tools optimize full structures, loops, or side-chains

Analysis of Structure and/or Sequence

Liability Prediction
- Epitope Scan
- Solubility Prediction
- Degradation Prediction
- Glycosylation
Model Analysis
- RosettaHoles
- Radius of Gyration
Interface Analysis